Cross-linked enzyme crystals of organophosphate hydrolase for electrochemical detection of organophosphorus compounds
نویسندگان
چکیده
منابع مشابه
Detection of organophosphorus compounds by covalently immobilized organophosphorus hydrolase.
As a consequence of organophosphorus (OP) toxins posing a threat to human life globally, organophosphorus hydrolase (OPH) has become the enzyme of choice to detoxify such compounds. Organophosphorus hydrolase was covalently immobilized onto a quartz substrate for utilization in paraoxon detection. The substrate was cleaned and modified prior to chemical attachment. Each modification step was mo...
متن کاملOrganophosphorus hydrolase-based assay for organophosphate pesticides
We report a rapid and versatile organophosphorus hydrolase (OPH)-based method for measurement of organophosphates. This assay is based on a substrate-dependent change in pH at the local vicinity of the enzyme. The pH change is monitored using fluorescein isothiocyanate (FITC), which is covalently immobilized to the enzyme. This method employs the use of poly(methyl methacrylate) beads to which ...
متن کاملAcetylcholinesterase Biosensors for Electrochemical Detection of Organophosphorus Compounds: A Review
The exponentially growing population, with limited resources, has exerted an intense pressure on the agriculture sector. In order to achieve high productivity the use of pesticide has increased up to many folds. These pesticides contain organophosphorus (OP) toxic compounds which interfere with the proper functioning of enzyme acetylcholinesterase (AChE) and finally affect the central nervous s...
متن کاملPhotonic crystal sensor for organophosphate nerve agents utilizing the organophosphorus hydrolase enzyme.
We developed an intelligent polymerized crystalline colloidal array (IPCCA) photonic crystal sensing material which reversibly senses the organophosphate compound methyl paraoxon at micromolar concentrations in aqueous solutions. A periodic array of colloidal particles is embedded in a poly-2-hydroxyethylacrylate hydrogel. The particle lattice spacing is such that the array Bragg-diffracts visi...
متن کاملEvaluation of the organophosphorus hydrolase enzyme activity in creams and investigation of its stability
The main purpose of this project is investigation of the organophosphorus hydrolase (OPH) enzyme activity in water in oil (w/o) and oil in water (o/w) creams and investigation of the OPH enzyme stability in formulated creams. OPH enzyme was extracted and purified from strain flavobacterium. The w/o and o/w creams were prepared using different formulations. In order to achieve an emulsion with m...
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ژورنال
عنوان ژورنال: World Journal of Microbiology and Biotechnology
سال: 2008
ISSN: 0959-3993,1573-0972
DOI: 10.1007/s11274-008-9851-y